Quantifying Nonspecific TEM β-Lactamase ( bla TEM ) Genes in a Wastewater Stream
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Quantifying Nonspecific TEM -Lactamase (blaTEM) Genes in a Wastewater Stream
To control the antibiotic resistance epidemic, it is necessary to understand the distribution of genetic material encoding antibiotic resistance in the environment and how anthropogenic inputs, such as wastewater, affect this distribution. Approximately two-thirds of antibiotics administered to humans are -lactams, for which the predominant bacterial resistance mechanism is hydrolysis by -lacta...
متن کاملA bla(TEM-1b)-derived TEM-6 beta-lactamase: a case of convergent evolution.
Since the first communication describing an organism that produces an enzyme capable of hydrolyzing extended-spectrum cephalosporins (5, 6), several outbreaks of Enterobacte-riaceae resistant to oxyimino--lactams have been reported. In these cases, the adopted survival strategy of bacteria challenged by the introduction of aztreonam, cefotaxime, ceftazi-dime, and other oxyimino--lactams was t...
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Background & objectives: Extended spectrum β-lactamases (ESBLs) are often plasmid mediated derived from mutations in the classic TEM and SHV genes by one or more amino acid substitution around the active site. Detection of TEM and SHV genes by molecular methods in ESBL producing bacteria and their pattern of antimicrobial resistance can provide useful information about its epidemiology and risk...
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A novel natural TEM beta-lactamase with extended-spectrum activity, TEM-138, was identified in a ceftazidime-resistant clinical isolate of Salmonella enterica serovar Infantis. Compared to TEM-1, TEM-138 contains the following mutations: E104K, N175I, and G238S. The bla(TEM-138) gene was located on a 50-kb transferable plasmid. Expression studies with Escherichia coli revealed efficient ceftazi...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2009
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01254-08